Curing plasmids from a strain

Yves Dessaux Yves.Dessaux at isv.cnrs-gif.fr
Tue Jun 10 04:54:15 EST 1997

In article <5n6ba2$nqk at info.abdn.ac.uk>, mbi071 at sysc.abdn.ac.uk says...

>Hi Martin,

>I've found that simply growing them in LB 2x overnight 
>cultures without antibiotic selection has done the trick without any 
>nasties. I just plated out, picked 100 colonies and replica plated them 
>on LB and LB Amp, about 20% had lost the plasmid.

This is exactly what I do, and it works great!

Good luck,


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