As a result of my enquiry regarding the curing of a plasmid from my
strain, I tried using a sub-inhibitory concentration of coumermycin
and obtained a >70% cure-rate. The plasmid was different to the
pBR322-based plasmid described in my earlier entry; this one is a low
copy-number derivative of pSC101, but the outcome was the same.
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Dr Martin Goldberg,
Dept. of Microbiology and Immunology,
University of Leicester,
University Road,
LEICESTER.
LE1 9HN
UK
Tel. +44 (0)116-252 3017
Fax. +44 (0)116-252 5030
E-mail mdg at le.ac.uk
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