> I'm looking for a polA mutant strain of E. coli to perform a gene
> knockout, and would appreciate any assistance in finding such a strain.
> I've looked in the ATCC catalogue, but without any cross-reference for
> genotypes it would take forever to look through all the strains.
In order to find a polA mutant strain of E.coli, i suggest you to
consult the "E. coli genetic stock center" web server
(http://cgsc.biology.yale.edu/cgsc.html) and to look for polA in the
category
"Gene Symbol Nameserver: Check for use as either Symbol or Synonym". In
this case you cill have the choice between 15 different strains carying a
polA mutation. In order to have the genotype of those strain choose anyone
and "click" on it.
I hope you will find the strain of your interest.
--
BREGEON Damien
Institut Jacques MONOD
2, Place Jussieu 75251 Paris cedex05
e-mail : bregeon at ijm.jussieu.fr
Tel : +33 (0)1 44 27 47 31
Fax : +33(0)1 44 27 57 16
