? on the types of evidence needed to determine if a bacteria is utilizing a compound

Karl Roberts kr1 at PGSTUMAIL.PG.CC.MD.US
Mon Oct 13 07:11:50 EST 1997

Dear Jeffrey,

	First, you are making life too hard for yourself (obscure hint).
Second, you really should not be asking for help on any kind of
examination.  Your own hard work and research will only show through if
you do this work yourself.  Sorry I can't be of more help.
Dr. Karl J. Roberts

 On 11 Oct 1997,
Jeffrey Grunner wrote:

> I am taking Microbiology 220 at ASU and I have a question for anyone out
> there who cares to reply.  The class was asked a question for a take home
> test and this is it: Supposing that you have a bacterium that is capable of
> utilizing a compound, called compound X, as a carbon and energy source,
> what types of evidence would show that the bacterium is actually growing on
> the compound X.  What types of positive and negative control experiments
> would be needed?
> I had a few thoughts on this, maybe you could use radiolabels on the
> coumpound x and then measure the amount of compound X which is utilized by
> the bacterium, but what could be used as a negative control experiment,
> could you use a different compound, or would you want to use a different
> bacterium that also utilizes the compound X.  Any help would be greatly
> appreciated.
> Thanks! 

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