Methanol Fixation and Acridine Orange

Barry H. Hellman, Jr. barryh at norfolk.infi.net
Mon Oct 13 20:17:55 EST 1997

I am not aware of AO being affected by methanol fixation although I 
usually use a Hotchkiss McManus Schiff's stain for image analysis to 
dtermine ploidy (which I assume you are doing?). You might like to use it 
to cross check your results although I assume you are already using a 
control. We routinely used 50% methanol for fixing our urine cytology 
specimens for DNA image analysis

Barry Hellman, MD
Bon Secours DePaul Medical Center
Norfolk, VA  USA

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