Klein Lab (merker01 at mcrcr.med.nyu.edu) wrote:
: Hello
: I am trying to make a plasmid with two ColE1 replication origins. Is
: this possible? Can E. coli maintain a plasmid with two of these
: origins? Has anybody ever been able to do this?
Yes. Yes. Yes (many people, including me).
Best wishes,
--
Chris Boyd | from, but not \ MRC Human Genetics Unit,
chrisb at hgu.mrc.ac.uk | on behalf of / Western General Hospital,
http://www.hgu.mrc.ac.uk/Users/Christopher.Boyd \ Edinburgh, EH4 2XU, SCOTLAND
