ColE1 replication origin

Chris Boyd chrisb at hgu.mrc.ac.uk
Tue May 5 10:42:50 EST 1998

Klein Lab (merker01 at mcrcr.med.nyu.edu) wrote:
: Hello

: I am trying to make a plasmid with two ColE1 replication origins.  Is
: this possible?  Can E. coli maintain a plasmid with two of these
: origins? Has anybody ever been able to do this?

Yes. Yes. Yes (many people, including me).

Best wishes,
Chris Boyd                      | from, but not \ MRC Human Genetics Unit,
chrisb at hgu.mrc.ac.uk            | on behalf of  /  Western General Hospital,
http://www.hgu.mrc.ac.uk/Users/Christopher.Boyd \   Edinburgh, EH4 2XU, SCOTLAND

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