Bacillus megaterium

Yersinia yersinia at CYBERNEX.NET
Wed May 6 16:19:36 EST 1998

Subject:     bacillus megaterium
Sent:        5/4/98 16:45
Received:    5/4/98 17:52
From:        @eatel.net
To:          microbio at net.bio.net

Halynic at eatel.net writes,

<I am looking for result to lab test to compare with mine to ensure they 

1. starch agar plate  - I got negative
2.spirit blue lipid agar plate- I got positive
3. nutriet gelatin- I got positive
4.fluid thioglycollate medium- I got aerobe
5.mr-vp -I got neg. on both
6.sim medium-neg. on all three
7.litmus milk-acid
8.simmons citrate agar slant-pos.  pH<7.6
9.phenol red glucese-acid
10.                lactose broth- acid
11.                mannitol -no acid

if any answers are known please let me know.>

A  no-longer-in-print handbook originally published in 1973 by the USDA - 
Agriculture Handbook 427, "Bacillus") - has a Bacillus speciation scheme 
(Key 1: A Stepping-Stone for Identifying Typical Strains of Bacillus 
Species, on page 97).  

According to this Key, B. megaterium is identified by the following 

Catalase positive, Voges-Proskauer negative,  no growth at 65C,  negative 
for starch hydrolysis,  negative for acid and gas from glucose, and 
positive for citrate utilization.

Since I'm at home now, I don't have a Bergey's Manual of Systematic 
Bacteriology accessible...but I'd suggest you compare your test results 
with those in Bergey's...or, consider if you're sure your bug is B. 


"If you're not part of the solution, you're part of the precipitate."

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