I'm working with a membrane protein of E.coli which is quite hydrophobic
and which I can't overproduce in sufficient amounts for purification and
antibody production. So I'm trying to find special peptide-sequences of
the protein for use as antigens.
Now my questions:
Does anyone out there know anything about the properties such a peptide
sequence has to have to be suitable as an antigen? Or which amino acids
shouldn'd be present at all? Are there any algorithms for calculating the
"antigenicity" of a peptide? Are there any servers/programs in the net to
get informations from?
Thanks for your help!