busaya apichaisataienchote wrote:
>>>> Dear all,
>> Does anyone know how to do purify chitinases from Pseudonocardia spp. properly?
>> I tried to use ammonium sulfate to precipitate protein firstly,then dissolve in distilled
>> water and dialysis against Tris -HCl . After detection chitinase by p-DAB method,we
> found negative result even this culture showed clear zone on chitin agar medium
>> widely after 4 days of incubation time.
>> Any suggestions please don't hesitate to reply me :busaya at kanate.su.ac.th>>>> Free web-based email, Forever, From anywhere!
What about running solution through a chitin column?