Does anyone know how to do purify chitinases from Pseudonocardia spp. properly?
I tried to use ammonium sulfate to precipitate protein firstly,then dissolve in distilled
water and dialysis against Tris -HCl . After detection chitinase by p-DAB method,we
found negative result even this culture showed clear zone on chitin agar medium
widely after 4 days of incubation time.
Any suggestions please don't hesitate to reply me :busaya at kanate.su.ac.th
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