IUBio

E.coli + luciferase

Robert A. Monticello, Ph.D. rmonti at wolv.tdsnet.com
Mon May 11 12:03:00 EST 1998


I have some experience with this and I don't think you will find an E.
coli strain that synthsizes FF luciferase and its probably not what your
looking for anyway.  Try looking up the ATP Assay Kit made by LKB Wallac. 
You should be able to measure total ATP concentration in whole or lysed
cells with this. hope it helps, Bob

In article <6ikalc$g0b$3 at kanja.arnes.si>, "Alexis Zrimec"
<alexis.zrimec at guest.arnes.si> wrote:

> Hello,
> 
> I'm working on a project of measuring the ATP pool in Escherichia coli cells
> and I'm interested in E.coli cells that can synthetize the firefly
> luciferase. Where could I find these strains?
> 
> Thank you for your help,
> 
> Alexis Zrimec



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