embedding media for thinsectioning?

Robert Witzig tatonka at cs.tu-berlin.de
Tue Jun 1 14:10:25 EST 1999

Hello there,

I´m interested in information about embedding techniques for
thinsectioning of biofilms.
For my purposes the embedding medium should meet the following demands:

it should
+ be good soluble in water,
+ allow for subsequent staining procedures like Alcian-blue staining,
+ be stable under treatment with ethanol,
+ be applicable in fluorescence microscopy, therefore not showing
+ be variable in consistence,
+ allow for microtome thinsectioning to thicknesses of about 10µm.

Thank you for your help

Robert Witzig
Technical University Berlin

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