Growth media for bacteria

Dr.Abhay Shendye abt at PN3.VSNL.NET.IN
Thu Jun 3 04:12:20 EST 1999

Dear Sheinach,

I do not know whether T can fully satisfy you with this answer.

The rate of any biochemical reaction is dependent [and mostly proportional] to
the concentration of the substrate. So, this answers your 10 fold dilution

The 10 fold higher may negatively affect due to its osmotic pressure.

However, recently I have come across some work [unpublished] wherein the
organisms show opt growth rates at 1/100 - 1/1000 dilution of media containing
1% glucose. The ongoing work suggests that these may be totally new species
not cultivated so far!

Dr. Abhay Shendye

Sheinach Dunn wrote:

> Could someone please tell me the answer and where I can verify it for the
> following question:
> Explain why growth of many bacteria would be negatively affected both by a
> 10-fold increase and a 10-fold decrease for a 4% glucose concentration
> media?
> Starvation and inhibition are the two reasons I came up with but I don't
> know where to look to verify or discount my answers.
> please reply to ratz at clear.net.nz

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