Graham Shepherd wrote:
>> Jesse Stricker wrote in message ...
> >
> > Hey, everybody. I'm looking for some big prokaryotes. I've been
> >doing light microscopy with E.coli-sized cells, and I'd like something
> >bigger -- as big as I can get. I know about Thiomargarita and
> >Epulopiscium, but neither of those will grow in pure culture. I'd like
> >something that I can grow in a moderately equipped microbiology lab. Any
> >ideas?
> >
> > Thanks,
> >
> > Jesse Stricker
> >
> >--
> > Jesse Stricker jds19 at acpub.duke.edu>> >
>> Bacillus megatherium (if it's still called that)
>> GS
Myxococcus xanthus is relatively large compared to other bacteria. The
myxobacteria in general are quite fascinating (developmental
characteristics, eukaryotic-like properties). They also have the
largest genomes known for prokaryotes (8-13 Mbp, about 2-3X that of E.
coli).
M. xanthus grows well in a Casitone medium (1-2%) buffered to pH of
7.2-7.6. They are slow-growers, however; gen. time is about 3.5-5
hrs.
If you have access to the latest edition of "The Prokaryotes, I would
higly recommend the chapter that on the myxobacteria. They have every
media formulation necessary.
--
C. J. Fields
Graduate Student, Dept. of Biological Sciences
The University of North Texas
Denton, TX
email : cjfields at jove.acs.unt.edu
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