Large prokaryotes

Chris Fields cjfields at jove.acs.unt.edu
Sun Jun 6 01:51:03 EST 1999

Graham Shepherd wrote:
> Jesse Stricker wrote in message ...
> >
> > Hey, everybody.  I'm looking for some big prokaryotes.  I've been
> >doing light microscopy with E.coli-sized cells, and I'd like something
> >bigger -- as big as I can get.  I know about Thiomargarita and
> >Epulopiscium, but neither of those will grow in pure culture.  I'd like
> >something that I can grow in a moderately equipped microbiology lab.  Any
> >ideas?
> >
> > Thanks,
> >
> > Jesse Stricker
> >
> >--
> > Jesse Stricker jds19 at acpub.duke.edu
> >
> Bacillus megatherium (if it's still called that)
> GS

Myxococcus xanthus is relatively large compared to other bacteria.  The
myxobacteria in general are quite fascinating (developmental
characteristics, eukaryotic-like properties).  They also have the
largest genomes known for prokaryotes (8-13 Mbp, about 2-3X that of E.

M. xanthus grows well in a Casitone medium (1-2%) buffered to pH of
7.2-7.6.  They are slow-growers, however; gen. time is about 3.5-5

If you have access to the latest edition of "The Prokaryotes,  I would
higly recommend the chapter that on the myxobacteria.  They have every
media formulation necessary.

C. J. Fields
Graduate Student, Dept. of Biological Sciences
The University of North Texas
Denton, TX 

email : cjfields at jove.acs.unt.edu
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