Erythrocyte ghosts

[William J. Mason]

I am curious if any of you know or have a protocol for creating
erythrocyte ghosts.  These are RBCs that have been gently lysed to remove
the hemoglobin and then "resealed" for purposes of studying the plasma
memb.  

Also, would any of you have a good protocol for separating bacteria from
blood cells (ie. bacteria from a blood culture).  Centrifugation at varied
speeds and times typically sediments most of the bacteria with the RBCs.  

Thanks for your help,

Jeff Mason
University of Arkansas, Biological Sciences/Microbiology
wmason at comp.uark.edu
http://comp.uark.edu/~wmason