Erythrocyte ghosts

[Pierre Juteau]

Concerning separation of blood cells and bacteria, a self-generating density
gradient that could be created with Percoll (Pharmacia) or similar products
should work

Pierre

"William J. Mason" wrote:

> I am curious if any of you know or have a protocol for creating
> erythrocyte ghosts.  These are RBCs that have been gently lysed to remove
> the hemoglobin and then "resealed" for purposes of studying the plasma
> memb.
>
> Also, would any of you have a good protocol for separating bacteria from
> blood cells (ie. bacteria from a blood culture).  Centrifugation at varied
> speeds and times typically sediments most of the bacteria with the RBCs.
>
> Thanks for your help,
>
> Jeff Mason
> University of Arkansas, Biological Sciences/Microbiology
> wmason at comp.uark.edu
> http://comp.uark.edu/~wmason

--
****************************************************
Pierre Juteau
Associé de recherche
INRS - Institut Armand-Frappier
Groupe de microbiologie de l'environnement
531, des Prairies boulevard
Laval (QC)
Canada
H7V 1B7

tél: (450) 687-5010, ext.4400
fax: (450) 686-5501

mailto:pierre.juteau at inrs-iaf.uquebec.ca
http://www.inrs-iaf.uquebec.ca