I work with lactobacilli and I use the MRS medium (Difco laboratories, Detroit, Mi, USA). This is a medium widely applied to lactobacilli growth (See: De Man, Rogosa and Sharpe, 1960, Journal of Applied Bacteriology, vol.23, pp.130-135). If you only want to grow the lactobacilli, it's an adequate medium. But if you want to isolate some bacterial products, like expolysaccharides, you must have to use a mineral medium. It is possible to identify lactobacilli species by differential plating,if you don't know which strain you're working with (See: Charteris et al., 1997, International Journal of Food Microbiology, vol.35, pp.1-27). I hope these informations will help you!
ianj <ianj at zipworld.com.au> a écrit dans le message : 8n8hki$sau$1 at nina.pacific.net.au...
The speciation of lactobacillus, as described in Bergey's manual can be performed using the API 50 CHL kit supplied by Biomerieux. It's a little expensive- but so is purchasing all those sugars and makign up the solutions!
"Ivano" wrote in message <001501c003b9$d4882860$d30a569d at 86.10.5.alpha.incqs.fiocruz.br>...
I need information how to identify Lactobacillus species. I´ve seraching on the Bergey´s manual, but I need to know the culture media base for sugars, aminoacids, etc.
Thank you in advance.
Ivano de Filippis
FUNDAÇÃO OSWALDO CRUZ
Instituto Nacional de Controle de Qualidade em Saúde - INCQS
Depto de Microbiologia - Bloco 08
Laboratório de Materiais de Referência
Av. Brasil, 4365 - Manguinhos
Rio de Janeiro-RJ - CEP 21045-900
E-mail: ivano at alpha.incqs.fiocruz.brivano at gene.dbbm.fiocruz.brivano at osite.com.br
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