How do you extract DNA from a polyacrylamide gel?
I run DGGE gels and need to excise the bands for PCR reamplification and
sequencing. I've tried cut out bands and freeze/thaw them ×3 in water but
didn't get
any product after the following PCR. Please, let me know if you know of a
good
method to recover the DNA.
Thank you
Lise Larsen
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