Hi
I used forward and reverse primers derived from conserved sequences
available in literature to hook out a particular gene.I got a single
amplified product of 1.4kb using both the primers together as well as
using the reverse primer alone, but didn't get any amplification using
the forward primer alone.I want to know whether it is possible to hook
out the full gene by using the single primer or can it be a part of the
gene or what?Thanks
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