Looks like you are amplifying something that is flanked by inverted repeats
representing the reverese primer sequence. The only way to get any
meaningful information from this is to sequence the amplified product
(which should be done in any case as a logical next step, regardless of the
amplifying primer).
At 02:51 PM 8/27/00 GMT, parvaizqazi at my-deja.com wrote:
>Hi
>I used forward and reverse primers derived from conserved sequences
>available in literature to hook out a particular gene.I got a single
>amplified product of 1.4kb using both the primers together as well as
>using the reverse primer alone, but didn't get any amplification using
>the forward primer alone.I want to know whether it is possible to hook
>out the full gene by using the single primer or can it be a part of the
>gene or what?Thanks
>parvaizqazi
>>>>>Sent via Deja.com http://www.deja.com/>Before you buy.
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Dept. of Molecular Biology
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