Staining of flagella

Emir Khatipov khatipovNO at NOuchicago.edu
Tue Oct 23 20:16:40 EST 2001

I don't know of any fluorescent staining methods, but I do remember that:
1) in many cases flagella can be seen under polarized light - if the bugs
are too motile, you can try making a slide with 0.7% agar
2) careful heat fixation  (or fixation in Os vapors) and staining with
protein-binding dyes like crystal violet allows to see flagella, too.

Otherwise check Methods of Mol. Bacteriology, Ed. Gerhardt (don't remember
year, might be wrong with the title, too, but you can find it in


"gerhard" <gerne1 at hotmail.com> wrote in message
news:4005dc5d.0109071943.2d7d4c24 at posting.google.com...
> Hi everyone
> I would like to detect the presence of flagella on bacteria,
> preferentially by fluorescence.
> Does anyone know of a flagellum specific fluorescent stain, perhaps an
> antibody that recognises flagella of a variety of species?
> Thanks in advance
> Gerhard Nebe-von-Caron

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