Staining of flagella

David Faguy dfaguy at unm.edu
Wed Oct 24 12:12:31 EST 2001

Hello, I missed the original post, but check out:
Real-Time Imaging of Fluorescent Flagellar Filaments Turner et al. J.
Bacteriol. 182:2793 (2000)
keep your wallet handy: the dyes they used aren't cheap.

"Emir Khatipov" <khatipovNO at NOuchicago.edu> wrote in message
news:VCoB7.130$N4.4377 at news.uchicago.edu...
> I don't know of any fluorescent staining methods, but I do remember that:
> 1) in many cases flagella can be seen under polarized light - if the bugs
> are too motile, you can try making a slide with 0.7% agar
> 2) careful heat fixation  (or fixation in Os vapors) and staining with
> protein-binding dyes like crystal violet allows to see flagella, too.
> Otherwise check Methods of Mol. Bacteriology, Ed. Gerhardt (don't remember
> year, might be wrong with the title, too, but you can find it in
> Amazon.com).
> Emir
> "gerhard" <gerne1 at hotmail.com> wrote in message
> news:4005dc5d.0109071943.2d7d4c24 at posting.google.com...
> > Hi everyone
> >
> > I would like to detect the presence of flagella on bacteria,
> > preferentially by fluorescence.
> >
> > Does anyone know of a flagellum specific fluorescent stain, perhaps an
> > antibody that recognises flagella of a variety of species?
> >
> > Thanks in advance
> >
> > Gerhard Nebe-von-Caron

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