Liefson's (?spelling) flagella stain is a fuschin based stain and is VERY
difficult to get a good stain. The glass slides must be super clean, the
organisms must be a young culture and diluted to the right concentration.
Any agitation in the dilution process or even in the smearing can dislodge
the flagella.
--
John Gentile MS M(ASCP)
Laboratory Information, QA Manager
VA Medical Center
Providence, RI
yjgent at home.com
> From: "Kevin Carter" <K.Carter at bham.ac.uk>
> Organization: The University of Birmingham news server
> Newsgroups:
>sci.bio.microbiology,bionet.microbiology,sci.bio.immunocytochem,sci.bio.ecol
og> y
> Date: Wed, 24 Oct 2001 13:52:17 +0100
> Subject: Re: Staining of flagella
>> Isn't acid fast staining ok for flagella
> KC
>> "Emir Khatipov" <khatipovNO at NOuchicago.edu> wrote in message
> news:VCoB7.130$N4.4377 at news.uchicago.edu...>> I don't know of any fluorescent staining methods, but I do remember that:
>> 1) in many cases flagella can be seen under polarized light - if the bugs
>> are too motile, you can try making a slide with 0.7% agar
>> 2) careful heat fixation (or fixation in Os vapors) and staining with
>> protein-binding dyes like crystal violet allows to see flagella, too.
>>>> Otherwise check Methods of Mol. Bacteriology, Ed. Gerhardt (don't remember
>> year, might be wrong with the title, too, but you can find it in
>> Amazon.com).
>>>> Emir
>>>>>> "gerhard" <gerne1 at hotmail.com> wrote in message
>> news:4005dc5d.0109071943.2d7d4c24 at posting.google.com...>>> Hi everyone
>>>>>> I would like to detect the presence of flagella on bacteria,
>>> preferentially by fluorescence.
>>>>>> Does anyone know of a flagellum specific fluorescent stain, perhaps an
>>> antibody that recognises flagella of a variety of species?
>>>>>> Thanks in advance
>>>>>> Gerhard Nebe-von-Caron
>>>>>>
