IUBio

ESBL testing

Tracy Shorts tracy.shorts at ntlworld.com
Sun Oct 28 19:26:26 EST 2001


I work in hospital microbiology lab in the UK.  We screen for ESBL's
by the antibiogram, noting a "notch" between the augmentin and ceftriaxone
disks on a routine sensitivity plate (we use stokes method).  Then we
cornfirm ESBL's by using the Mast Diagnostics ESBL kit (compares zones sizes
of 3 third gen. cefalosporins discs to discs containing the same
cefalosporin + clavulanic acid).  We have found that organsims other than
E.coli and Kleb spp. can be ESBL's.

Hope this is of help.






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