separating bacteria from skim milk

Thirunavukkarasu Annamalai a_thiruna at hotmail.com
Tue Dec 31 15:35:13 EST 2002


i am wondering whether anyone could kindly help me with the following

I inoculate milk samples (mainly skim milk) with about 10^7 to 10^8
CFU/ml of gram negative bacteria and then after certin hours of
incubation , i have to collect bacterial cells for further
manipulations. Is there any method to remove the bacterial  cells
alone without  getting the other  components of milk, or atleast to
get rid of most of the milk debris. i have tried different
centrifuging. if you have any info regarding some filters or also
about some centrifuge techniques, it would be great.



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