Richard Oeffner roeffner at csusm.edu
Wed May 15 11:51:04 EST 2002


I don't know if this would be of any help to you , but BD Biosciences makes an
Oxygen sensitive fluorescing plate that we use to test inhibition of growth
from natural product extracts in our lab. It is a quick simple assay, and I can
get results from Mycobacterium marinum plates in 48 hours. The plates are about
$30 each, but you can run up to 40 samples in duplicate with controls.
I just wanted to see of this might be of some use to you.


Magix wrote:

> Hi all,
> Thanks for your responses.
> Actually, most of the time we don't know what the substance we are testing
> for is, especially when dealing with fish kills. We have been approached by
> suppliers to use Vibrio fischeri but the test kit is very expensive (both
> the machine and the recurrent costs) and it doesn't make sense to purchase
> one especially when we only get less than 10 incidents a year.  These kits
> require multiple dilutions and then calculations for an EC50.
> Others are much simpler with a single test and % inhibition calculation.
> I think they are all trying to confuse me!
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