Vladimir,
Use chloramphenicol NOT ampicillin (the bacterium is Cam-R). This seems to
be your problem.
Vincent Mulholland,
Molecular Biology Unit Manager,
Diagnostics & Molecular Biology Section,
Scottish Agricultural Science Agency,
East Craigs,
Edinburgh EH12 8NJ, U.K.
URL: http://www.sasa.gov.uk/
E-Mail: Vince.Mulholland at sasa.gsi.gov.uk
Hi,
BL21 CodonPlus (DE3) RIL from Stratagene has genotype E. coli B F(-) ompT
hsdS(rB(-) mB(-)) dcm(+) Tet(r) gal ?(DE3) endA Hte[argU ileY leuW Cam(r)],
and appears it should be able to grow in a minimal medium. We tried to grow
it in M9 with glucose (and ampicillin for plasmid maintenance) with no
avail. Tech. rep. from Stratagene was not of much help suggesting to add
proline (?), cysteine (?) and thiamine (?) with no guarantee to work. Any
advice what may be a problem and how to make it grow?
Thanks,
Vladimir Yamshchikov
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