On 22 May 2002, Vince Mulholland wrote:
> Vladimir,
>> Use chloramphenicol NOT ampicillin (the bacterium is Cam-R). This seems to
> be your problem.
>> Hi,
>> BL21 CodonPlus (DE3) RIL from Stratagene has genotype E. coli B F(-) ompT
> hsdS(rB(-) mB(-)) dcm(+) Tet(r) gal ?(DE3) endA Hte[argU ileY leuW Cam(r)],
> and appears it should be able to grow in a minimal medium. We tried to grow
> it in M9 with glucose (and ampicillin for plasmid maintenance) with no
> avail. Tech. rep. from Stratagene was not of much help suggesting to add
> proline (?), cysteine (?) and thiamine (?) with no guarantee to work. Any
> advice what may be a problem and how to make it grow?
>> Thanks,
>> Vladimir Yamshchikov
Vince, are you sure? Vladimair, what about the idea of spreading a
minimal plate with cells and putting a grain or two of all the amino
acids on your chemical shelf in sectors and see what makes a difference
(classical experiment). Regards, Mike.
