Advice in microbiology needed

Des O'Connor des at micro-search.com
Wed May 22 16:37:30 EST 2002

Is chloramphenicol carcinogenmic ?


"Vince Mulholland" <Vince.Mulholland at sasa.gsi.gov.uk> wrote in message
news:C39747D75908D31195EE0090273C23E9F41B62 at barra...
> Vladimir,
> Use chloramphenicol NOT ampicillin (the bacterium is Cam-R). This seems to
> be your problem.
> Vincent Mulholland,
> Molecular Biology Unit Manager,
> Diagnostics & Molecular Biology Section,
> Scottish Agricultural Science Agency,
> East Craigs,
> Edinburgh EH12 8NJ, U.K.
> URL:    http://www.sasa.gov.uk/
> E-Mail: Vince.Mulholland at sasa.gsi.gov.uk
> Hi,
> BL21 CodonPlus (DE3) RIL from Stratagene has genotype E. coli B F(-) ompT
> hsdS(rB(-) mB(-)) dcm(+) Tet(r) gal ?(DE3) endA Hte[argU ileY leuW
> and appears it should be able to grow in a minimal medium. We tried to
> it in M9 with glucose (and ampicillin for plasmid maintenance) with no
> avail. Tech. rep. from Stratagene was not of much help suggesting to add
> proline (?), cysteine (?) and thiamine (?) with no guarantee to work. Any
> advice what may be a problem and how to make it grow?
> Thanks,
> Vladimir Yamshchikov
> ---

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