Microbio Techniques

Mark Farrar micmdf at leeds.ac.uk
Sat May 25 07:46:20 EST 2002

The cutaneous propionibacteria although described as anaerobes are actually
microaerophilic. They can be manipulated under aerobic conditions but are
routinely grown and isolated from skin under anaerobic conditions. We use
reinforced clostridial agar supplemented with 0.1% (v/v) Tween 80 as a lipid
source and furazolidone (final conc. 6ug/ml) to inhibit growth of
staphylococci. For antibiotic testing we use the agar dilution method, usually
due to the large numbers of isolates to be tested.

Mark Farrar

JEDilworth wrote:

> What John has said is correct. To perform anaerobic micro you need a lot
> of stuff, and if you're starting from scratch, it will cost you some big
> bucks.
> Why do you want to do this, anyway? Propionibacterium sp. are anaerobes
> that live on the skin. Some labs just report tham as anaerobic
> diphtheroids, as there are many types of diphtheroid organisms living on
> the skin. They tend to contaminate blood cultures.
> Here's a link that will send you in many anaerobic directions:
> http://www.anaerobe.org/links.htm
> Judy Dilworth, M.T. (ASCP)
> Microbiology
> John Gentile wrote:
> >
> > In what context are you doing this? Are you in a well supplied lab or are
> > you doing this as a hobby?

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