Hi,
I'm growing yeast strain S288C (MATalpha trp1-0) in 2L fermentor. The
medium is YEPD and the setup is as follows:
- fermentation type: batch
- temperature: 30 'C
- initial pH: 4.8 (pH is not controlled)
- airflow (1.12 L/min) and stirring (300 rpm) are kept constant.
My "problem" is that I'm getting unusual pH- and pO2 curves compared on
what I have been used to in fermentations of typical bakers yeast.
Please, look the picture
http://hytti.uku.fi/~jpparvia/joutavia/growth_curve_s288c.png
I'm wondering what happens in about 20 hours after inoculation. There are
sharp changes on both curves. Could someone share an opinion?
Regard,
---------------
J Parviainen