From nojunk from knology.net Thu Nov 5 16:17:50 2009 From: nojunk from knology.net (amdx) Date: Thu Nov 5 17:54:15 2009 Subject: [Microbiology] Looking for Cell with unique Composition Message-ID: High School project, looking for something different. Do you know of any cells that I can research to make my project different than the usual. Need to make a 3D model. The Mav From bks from panix.com Thu Nov 5 16:36:30 2009 From: bks from panix.com (Bradley K. Sherman) Date: Thu Nov 5 17:54:19 2009 Subject: [Microbiology] Re: Looking for Cell with unique Composition References: Message-ID: In article , amdx wrote: >High School project, looking for something different. >Do you know of any cells that I can research to make >my project different than the usual. >Need to make a 3D model. > The Mav > > Surprisingly, each cell that exists, and has ever existed, has unique composition. Check out these cells: --bks From nojunk from knology.net Thu Nov 5 18:48:30 2009 From: nojunk from knology.net (amdx) Date: Thu Nov 5 19:26:59 2009 Subject: [Microbiology] Re: Looking for Cell with unique Composition References: Message-ID: "Bradley K. Sherman" wrote in message news:hcvggu$s8i$1@reader1.panix.com... > In article , > amdx wrote: >>High School project, looking for something different. >>Do you know of any cells that I can research to make >>my project different than the usual. >>Need to make a 3D model. >> The Mav >> >> > > Surprisingly, each cell that exists, and has ever existed, has > unique composition. > > Check out these cells: > > > --bks > Thanks Bradley, That's neat, looks so much like an insect walking on that bubble. Just so I'm clear, that is a single cell and the cilia are part of that cell. Many more on the site that I'll check out too. Thanks, The Mav From bks from panix.com Thu Nov 5 19:27:00 2009 From: bks from panix.com (Bradley K. Sherman) Date: Thu Nov 5 19:58:16 2009 Subject: [Microbiology] Re: Looking for Cell with unique Composition References: Message-ID: In article , amdx wrote: >Just so I'm clear, that is a single cell and the cilia are part of that >cell. > Now, now, don't presume on my good nature. --bks From vida_tafakori from yahoo.com Mon Nov 9 06:31:02 2009 From: vida_tafakori from yahoo.com (vida tafacori) Date: Mon Nov 9 14:15:02 2009 Subject: [Microbiology] a question Message-ID: <627318.10854.qm@web33301.mail.mud.yahoo.com> Dear colleagues I appreciate if anybody can help me in the following problem: I used Lpp'-ompA system (developed by Georgio et al., 1996) for surface display of a bacterial metallothioneine fused to chitin binding domain and 6Xhis-tag (on the surface of E.coli BL21). The length of this passenger protein is approximately 15kDa. We did metal binding assay using whole cell and we measured adsorption using a solution of cadmium Cd(NO3)2 in Tris-Hcl pH7.0. But it didn't show any adsorption. Hydropathy plot showed the passenger proteins are hydrophile and also it didn't show any transmembrane domain. I would like to know why does not this protein show any adsorption? I appreciate your help. Best Wishes Reza ? ? From H.Steen-Thornhammar from lse.ac.uk Tue Nov 10 10:30:15 2009 From: H.Steen-Thornhammar from lse.ac.uk (H.Steen-Thornhammar@lse.ac.uk) Date: Tue Nov 10 12:58:12 2009 Subject: [Microbiology] acetobacter xylinum - where can I get it? Message-ID: <23E26F0678A3FE4FA196D49190A5809402982BB3@EXCHS3.lse.ac.uk> Hi, My name is Hannes Steen Thornhammar and I am student at the London School of Economics. I am currently rsearching topics in sustainable futures and came across your name in regards to acetobacter xylinum. Would you be able to tell me how I can actually attain samples of the bacteria. Can I grow it myself? Can I buy it in the UK, London? Your assisstance in this matter would be greatly appreciated Best, Hannes Please access the attached hyperlink for an important electronic communications disclaimer: http://www.lse.ac.uk/collections/secretariat/legal/disclaimer.htm From yjgent from nospamcox.net Tue Nov 10 20:21:04 2009 From: yjgent from nospamcox.net (John Gentile) Date: Wed Nov 11 12:33:22 2009 Subject: [Microbiology] Re: acetobacter xylinum - where can I get it? References: Message-ID: <2009111020210416807-yjgent@nospamcoxnet> On 2009-11-10 10:30:15 -0500, said: > Hi, > > My name is Hannes Steen Thornhammar and I am student at the London > School of Economics. I am currently rsearching topics in sustainable > futures and came across your name in regards to acetobacter xylinum. > Would you be able to tell me how I can actually attain samples of the > bacteria. Can I grow it myself? Can I buy it in the UK, London? > > Your assisstance in this matter would be greatly appreciated > > Best, > > Hannes > > Please access the attached hyperlink for an important electronic > communications disclaimer: > http://www.lse.ac.uk/collections/secretariat/legal/disclaimer.htm Are you studying bacteriology or economics? Do you have any experience in handling bacteria? Do you have access to laboratory facilities that will allow you to manage a bacterial culture? Do you have a professor who is mentoring you? Handling bacteria can be a very dangerous proposition if not done properly. Even so called non-pathogenic bacteria can cause some kind of havoc if it gets into the wrong place. There are places where you can purchase bacterial stocks, but you have to be associated with a certified laboratory or educational facility with proper labs to handle it. How are you planning to pay for it? Lots of questions, hope you have a plan. -- John Gentile MS, M(ASCP) Laboratory Information Mgr. VA Medical Center Providence, RI yjgent@cox.net From Vince.Mulholland from sasa.gsi.gov.uk Wed Nov 11 09:29:09 2009 From: Vince.Mulholland from sasa.gsi.gov.uk (Vince Mulholland) Date: Wed Nov 11 12:33:32 2009 Subject: [Microbiology] acetobacter xylinum - where can I get it? Message-ID: Hannes, Do not try buying, or otherwise obtaining, a bacterial culture if you do not have the experience or facilities to grow it (responsible organisations would not give you a culture in any case). Find a local microbiologist who can help you. He/she will know how to obtain this bacterium and will have access to the laboratory facilities you need. A biologist at the LSE might know someone local who can help you. Best wishes, Vince Vincent Mulholland Molecular Biology Unit Manager - Diagnostics & Molecular Biology Section Science and Advice for Scottish Agriculture (SASA) SASA is a Division of the Scottish Government Rural Payments and Inspections Directorate Correspondents should note that all communications to or from SASA (Science and Advice for Scottish Agriculture ? a Division of the Rural Payments and Inspections Directorate of the Scottish Government) may be automatically logged, monitored and/or recorded for lawful purposes. The original of this email was scanned for viruses by the Government Secure Intranet (GSi) virus scanning service. On leaving the GSi this email was certified virus-free. From hmerker from ice.mpg.de Wed Nov 11 12:18:04 2009 From: hmerker from ice.mpg.de (Holger Merker) Date: Wed Nov 11 12:33:40 2009 Subject: [Microbiology] constitutive promoters needed Message-ID: <4AFAF1CC.6040307@ice.mpg.de> Dear all, I am looking for constitutive prokaryotic promoters (especially for E.coli, B.subtilis, Pseudomons fluorescens and putida, Acinetobacter) differing in their transcriptional activity except for the lac-promoter or the beta-lactamase promoter. I only found this webpage (http://margalit.huji.ac.il/) but I would like to know whether the specific promoter is a weak or strong promoter (beside the fact that it is constitutive). So, does anyone of you know a database containing such information? or could you suggest me some further constitutive promoters with regard to their transcriptional activity? Any suggestion/hint/comment is welcome. Thx a lot for your help. All the best, Holger -- Dipl.Biol. Holger Merker Max-Planck-Institut f?r Chemische ?kologie Abteilung f?r Bioorganische Chemie (Prof. Boland) Hans-Kn?ll-Strasse 8 07745 Jena Germany Tel.: +49-(0)3641-571212 From kv134369 from bcm.tmc.edu Wed Nov 11 13:11:39 2009 From: kv134369 from bcm.tmc.edu (Venken, Koen J. T.) Date: Wed Nov 11 16:06:28 2009 Subject: [Microbiology] constitutive promoters needed In-Reply-To: <4AFAF1CC.6040307@ice.mpg.de> References: <4AFAF1CC.6040307@ice.mpg.de> Message-ID: <0C5508B843D50F48A9406CB1ED65A9D4E18A94@BCMEVS12.ad.bcm.edu> I do not know of a database but you may want to look at (for E.coli at least): -Escherichia coli strains with promoter libraries constructed by Red/ET recombination pave the way for transcriptional fine-tuning. Braatsch S, Helmark S, Kranz H, Koebmann B, Jensen PR. Biotechniques. 2008 Sep;45(3):335-7. -The sequence of spacers between the consensus sequences modulates the strength of prokaryotic promoters. Jensen PR, Hammer K. Appl Environ Microbiol. 1998 Jan;64(1):82-7. -Construction and model-based analysis of a promoter library for E. coli: an indispensable tool for metabolic engineering. De Mey M, Maertens J, Lequeux GJ, Soetaert WK, Vandamme EJ. BMC Biotechnol. 2007 Jun 18;7:34. The first two papers include sequence info of the promoter sets. Best, Koen -----Original Message----- From: microbio-bounces@oat.bio.indiana.edu [mailto:microbio-bounces@oat.bio.indiana.edu] On Behalf Of Holger Merker Sent: Wednesday, November 11, 2009 11:18 AM To: microbio@magpie.bio.indiana.edu Subject: [Microbiology] constitutive promoters needed Dear all, I am looking for constitutive prokaryotic promoters (especially for E.coli, B.subtilis, Pseudomons fluorescens and putida, Acinetobacter) differing in their transcriptional activity except for the lac-promoter or the beta-lactamase promoter. I only found this webpage (http://margalit.huji.ac.il/) but I would like to know whether the specific promoter is a weak or strong promoter (beside the fact that it is constitutive). So, does anyone of you know a database containing such information? or could you suggest me some further constitutive promoters with regard to their transcriptional activity? Any suggestion/hint/comment is welcome. Thx a lot for your help. All the best, Holger -- Dipl.Biol. Holger Merker Max-Planck-Institut f?r Chemische ?kologie Abteilung f?r Bioorganische Chemie (Prof. Boland) Hans-Kn?ll-Strasse 8 07745 Jena Germany Tel.: +49-(0)3641-571212 _______________________________________________ Microbio mailing list Microbio@net.bio.net http://www.bio.net/biomail/listinfo/microbio From jbdundas from gmail.com Tue Nov 17 02:25:15 2009 From: jbdundas from gmail.com (jitesh dundas) Date: Tue Nov 17 11:43:36 2009 Subject: [Microbiology] Request For A partner on my projects In-Reply-To: <326ea8620911162324r1d0f4688y3d555301c6852855@mail.gmail.com> References: <326ea8620911162320r410a16b6ie8582c12bb1da100@mail.gmail.com> <326ea8620911162324r1d0f4688y3d555301c6852855@mail.gmail.com> Message-ID: <326ea8620911162325pa699e84x4d13283b5a05b694@mail.gmail.com> Dear Sir/Madam, I am looking for a research partner who could help me perform wet lab experiments. I do not have access to wet lab experiment equipments and that is the only thing that stops me from completing my work. I have 2 papers that need some biology inputs. Please let me know if there is any enthusiastic and willing scientist who is ready to help me in my work. I will be happy to make him my co-author in all the research papers that are involved ( for the current two papers and other papers in future too ) I look forward to a positive reply from some experts in this group. Regards, Jitesh Dundas http://jiteshbdundas.blogspot.com From lovey5dovey from hotmail.com Sun Nov 22 10:37:25 2009 From: lovey5dovey from hotmail.com (Shalini selvam) Date: Sun Nov 22 13:01:42 2009 Subject: [Microbiology] question Message-ID: Hello there!To anyone concern: I have a question. How long can a media last after being autoclaved and where should we store it?can we autoclave it twice? And how do we set up microbial consortia? Thank. Regards,Shalini _________________________________________________________________ Windows 7: Simplify what you do everyday. Find the right PC for you. http://windows.microsoft.com/shop From yjgent from nospamcox.net Sun Nov 22 21:16:51 2009 From: yjgent from nospamcox.net (John Gentile) Date: Sun Nov 22 22:31:16 2009 Subject: [Microbiology] Re: question References: Message-ID: <2009112221165116807-yjgent@nospamcoxnet> On 2009-11-22 10:37:25 -0500, Shalini selvam said: > > Hello there!To anyone concern: > > I have a question. > > How long can a media last after being autoclaved and where should we store > it?can we autoclave it twice? > > And how do we set up microbial consortia? > > Thank. > > Regards,Shalini > > _________________________________________________________________ > Windows 7: Simplify what you do everyday. Find the right PC for you. > http://windows.microsoft.com/shop Generally media that has been dispensed into screw capped tubes will last a lot longer, especially if the caps are screwed tight after the media has cooled. Petri dish media dries out and needs to be sealed in a bag or container and usually requires refrigeration. If you add blood to a media, it will shorten the "expriation date" significantly. I wouldn't trust blood media past 4 to 6 weeks. If you make the media in large flasks, you must cool the agar to about 50C and then pour into whaterver vial or plate you intend to use. It is not recommended to autoclave again since that could carmalize some of the sugars and may change some chemical properties and pH. You can remelt media by bringing it above the melting temp of agar which is around 97C, but don't go over that, just enough to get it all to melt. There are a few good references for media preparation including the Difco Manual and the American Society of Micribiology Manual of Clinical Micribiology. These are the references I've used in my many years of media making and storing. -- John Gentile MS, M(ASCP) Laboratory Information Mgr. VA Medical Center Providence, RI yjgent@cox.net From lovey5dovey from hotmail.com Mon Nov 23 04:15:58 2009 From: lovey5dovey from hotmail.com (Shalini selvam) Date: Mon Nov 23 12:27:19 2009 Subject: [Microbiology] question Message-ID: Hello there! Thank you Mr John and Bob for the previous reply. Regarding the question on how to construct microbial consortia, im trying to construct microbial consortia for bioremediation of hydrocarbon contaminated soil. All the papers that i came across grew the inoculum separately and then combined them. Can we combine them directly and then test for the abiltiy of bacteria to degrade oil ? Eg lets say flask 1 contains 1ml oil+100ml media+2ml inoculum of one type and flask 2 contains 1ml oil+100ml media+ 1ml each of 2 different inoculum for the testing? Thank you! Sincerely,Shalini _________________________________________________________________ New Windows 7: Simplify what you do everyday. Find the right PC for you. http://windows.microsoft.com/shop From finch.enteract from sbcglobal.net Mon Nov 23 17:42:56 2009 From: finch.enteract from sbcglobal.net (Deirdre Sholto Douglas) Date: Mon Nov 23 19:15:33 2009 Subject: [Microbiology] Re: question In-Reply-To: References: Message-ID: <7n0hfiF3a463eU1@mid.individual.net> Shalini selvam wrote: > > Hello there! > > Thank you Mr John and Bob for the previous reply. > > Regarding the question on how to construct microbial consortia, im trying to > construct microbial consortia for bioremediation of hydrocarbon > contaminated soil. All the papers that i came across grew the inoculum > separately and then combined them. What microbes comprise your consortium? Or rather, which are you planning to use? Contrary to popular belief, microbes aren't plug-and-play, things like competition for e- donors/acceptors comes into play once you leave the realm of the Pure Culture... there's considerably more to the process than simply bunging bugs in a bottle and awaiting results. > Can we combine them directly and then test for the abiltiy of bacteria to degrade oil ? Eg lets > say flask 1 contains 1ml oil+100ml media+2ml inoculum of one type and > flask 2 contains 1ml oil+100ml media+ 1ml each of 2 different inoculum > for the testing? Hydrocarbons (which includes the volatile organics) in general or oil, in particular? And if only oil, then what sort of oil? Crude, fuel, clean, dirty, high or low sulphur content? IMO, the experiment you propose has a lot of variables which have to be wrangled into submission before you'll get anything like a reproducible result. I wish you the best, however. Deirdre