From yjgent from nospamcox.net Thu Sep 3 19:27:04 2009 From: yjgent from nospamcox.net (John Gentile) Date: Thu Sep 3 21:18:04 2009 Subject: [Microbiology] Re: Do bacteria cause nasal mucus to smell bad? References: Message-ID: <2009090320270416807-yjgent@nospamcoxnet> On 2009-09-02 23:19:23 -0400, GreenXenon said: > Hi: > > Are there any bacteria that feed on nasal mucus? If so, do they > produce any colors and odors as a resulting of feeding on the mucus? > Will it smell like heated stale Swiss cheese? What color? When > bacteria feed on mucus, do they produce any slimy substances of their > own? Also, if mucus passes through the colon, will the fecal bacteria > feed on it? > > > Thanks > > P.S. These are not homework questions. These are questions of my own > interest. Most people do not realize that there are more bacterial cells in and on our bodies than there are human cells. There is a large population of bacteria that exist in all the mucus cavities, most of which are there that are a benefit to the host (you). But you can take these same bacteria and get them into an area where they do not usually belong and you may then have an infection. Usually if you have a smelly, pus & mucus discharge in the nasal cavities it is coming from one of the nasal sinuses. Sometimes a sinus can be blocked by swelling from a virus or an allergy and the normal mucus flow is backed up allowing the trapped bacteria to multiply and cause an immune reaction of pus cells to converge and try to kill the bacteria. If you are having these kinds of symptoms you should see a doctor - most primary care docs can easily look into your nasal cavity and identify the problem and prescribe the correct treatment. The last time I had a bout of sinusitis they gave me an antibiotic (to kill the bacteria) and a decongestant (to open the clogged passages). I can't tell from your description what kind of bacteria you are talking about - there are hundreds of kinds of bacteria that we all live with! To identify it would take a complete culture workup from the infected site. It is often difficult to separate the "normal" bacteria from the "infectious" bacteria, but that is what our real job is all about. -- John Gentile MS, M(ASCP) Laboratory Information Mgr. VA Medical Center Providence, RI yjgent@cox.net From cbenson from nutritionallabs.com Tue Sep 8 17:52:12 2009 From: cbenson from nutritionallabs.com (Christina Benson) Date: Tue Sep 8 21:23:38 2009 Subject: [Microbiology] USP Microbial Limits Prep Test Message-ID: <24031E4AE32244F98F946FA60DED23FC@NLNT> Hi there, I was wondering if you would be willing to share the SOP you wrote on how to perform the prep test? I am having a hard time figuring it out. I also think it is kind of an oxymoron to be testing antimicrobial stuff. Antimicrobial is good, right? Why ruin it by neutralizing? Thanks for your time, Christina Benson From jorge1907 from aol.com Wed Sep 9 16:24:35 2009 From: jorge1907 from aol.com (jorge1907@aol.com) Date: Wed Sep 9 17:04:08 2009 Subject: [Microbiology] Re: Microbio Digest, Vol 52, Issue 2 In-Reply-To: <200909091705.n89H5Op12565@net.bio.net> References: <200909091705.n89H5Op12565@net.bio.net> Message-ID: <8CBFFAB1067752F-2DD8-3A49A@webmail-m069.sysops.aol.com> What dio you mean "prep" test??? Neutralization is intended to allow detection of viable by not growing bugs. -----Original Message----- From: microbio-request@oat.bio.indiana.edu To: microbio@magpie.bio.indiana.edu Sent: Wed, Sep 9, 2009 1:05 pm Subject: Microbio Digest, Vol 52, Issue 2 Send Microbio mailing list submissions to microbio@net.bio.net To subscribe or unsubscribe via the World Wide Web, visit http://www.bio.net/biomail/listinfo/microbio or, via email, send a message with subject or body 'help' to microbio-request@net.bio.net You can reach the person managing the list at microbio-owner@net.bio.net When replying, please edit your Subject line so it is more specific than "Re: Contents of Microbio digest..." Today's Topics: 1. USP Microbial Limits Prep Test (Christina Benson) ---------------------------------------------------------------------- Message: 1 Date: Tue, 8 Sep 2009 16:52:12 -0600 From: "Christina Benson" Subject: [Microbiology] USP Microbial Limits Prep Test To: Message-ID: <24031E4AE32244F98F946FA60DED23FC@NLNT> Content-Type: text/plain; charset="us-ascii" Hi there, I was wondering if you would be willing to share the SOP you wrote on how to perform the prep test? I am having a hard time figuring it out. I also think it is kind of an oxymoron to be testing antimicrobial stuff. Antimicrobial is good, right? Why ruin it by neutralizing? Thanks for your time, Christina Benson ------------------------------ _______________________________________________ Microbio mailing list Microbio@net.bio.net http://www.bio.net/biomail/listinfo/microbio End of Microbio Digest, Vol 52, Issue 2 *************************************** From labcat76 from yahoo.com Wed Sep 9 18:42:53 2009 From: labcat76 from yahoo.com (Kate Hawley) Date: Wed Sep 9 19:57:14 2009 Subject: [Microbiology] Re: USP Microbial Limits Prep Test (Christina Benson) Message-ID: <787873.67102.qm@web52907.mail.re2.yahoo.com> Christina:In testing antimicrobials, neutralization should always be carried out to define the testing period. ?For example, you are looking at bleach and wonder how many bacteria are killed in a 15 second contact period. ?You expose the bacteria to the bleach for 15 seconds, then recover them in neutralizer. ?This stops the action of the bleach, the test period is defined as 15 seconds. ?Then plate the solution and wait for the 24 to 48 hours to see how many organisms survived and calculated your reduction from the inoculum (or zero time) organism count. ?If you had recovered the organisms in phosphate buffer or water, the bleach would still be acting on the organisms over the period required to enumerate the bacteria and your test period now is undefined or at best the whole 24 to 48 hours it takes to look at the plates. ?Regardless of the antimicrobial, whether fast acting like bleach/disinfectants or slower like silver or triclosan, neutralization is required for understanding kill times. ?Common neutralizers include letheen broth, D/E broth, and SCDLP, but you need to determine the appropriate neutralizer for your antimicrobial. Katherine Harrell Hawley Message: 1 Date: Tue, 8 Sep 2009 16:52:12 -0600 From: "Christina Benson" Subject: [Microbiology] USP Microbial Limits Prep Test To: Message-ID: <24031E4AE32244F98F946FA60DED23FC@NLNT> Content-Type: text/plain;??? charset="us-ascii" Hi there, I was wondering if you would be willing to share the SOP you wrote on how to perform the prep test?? I am having a hard time figuring it out.? I also think it is kind of an oxymoron to be testing antimicrobial stuff. Antimicrobial is good, right?? Why ruin it by neutralizing? Thanks for your time, Christina Benson From sajeesh.kappachery from gmail.com Thu Sep 10 04:06:58 2009 From: sajeesh.kappachery from gmail.com (sajeesh k) Date: Thu Sep 10 10:27:42 2009 Subject: [Microbiology] Can any body give some suggetion about growing biofilm on RO membrane Message-ID: <1e0abc6a0909100206o190e83feg839e8cf4e58d013@mail.gmail.com> Respected Reserchers, I am trying to set a chemostat experimental set up using CDC reacter for growing Aeromonas hydrophila biofilm on Reverse osmosis membrane. Can any body give suggetion about the strength of LB media sutable for the experimnet and the dilution facter.Also how long i shuld run the chemostat to get a robust biofilm on RO surface. Thaking you in advance, Sajeesh From subhash.g.venkat from gmail.com Thu Sep 10 01:32:50 2009 From: subhash.g.venkat from gmail.com (subhash subhash) Date: Thu Sep 10 10:29:08 2009 Subject: [Microbiology] hai Message-ID: <666de2570909092332w5b3f5392rd562894942c21257@mail.gmail.com> sirs and madams here i have one problem with cy3 cy5 fluorescent images they show only light imaging cntrovercy during epifluorescent microscopic observation.i cont differentiate the cy5 images from cy3 images. cy5 images are not visualised under this epifluorescent microscope, so please help to me reduce such problems during visualisation. if possible explain with diagrams. with regards subhash.g.venkat From jorge1907 from aol.com Fri Sep 11 06:33:01 2009 From: jorge1907 from aol.com (jorge1907@aol.com) Date: Fri Sep 11 10:49:58 2009 Subject: [Microbiology] Re: Microbio Digest, Vol 52, Issue 3 In-Reply-To: <200909101704.n8AH4Jp18038@net.bio.net> References: <200909101704.n8AH4Jp18038@net.bio.net> Message-ID: <8CC00EAC0C623F3-7BB8-25A37@webmail-d065.sysops.aol.com> Katherine, USP has nothing to do with the level of activity characterized by disnfectants.? The concept the USP attempts to address is the carry-over into culture of sufficient preservative that the viable microbes don't grow.? Preservatives, esp. parabens, don't kill as rapidly as disinfecatants - rather they inhibit and the bugs die off.? Success crtieria established by USP specify kill - so one must ensure that the bugs are dead. It's not clear what the original poster wanted in the "prep test.".? The USP is pretty simple to follow. -----Original Message----- From: microbio-request@oat.bio.indiana.edu To: microbio@magpie.bio.indiana.edu Sent: Thu, Sep 10, 2009 1:04 pm Subject: Microbio Digest, Vol 52, Issue 3 Send Microbio mailing list submissions to microbio@net.bio.net To subscribe or unsubscribe via the World Wide Web, visit http://www.bio.net/biomail/listinfo/microbio or, via email, send a message with subject or body 'help' to microbio-request@net.bio.net You can reach the person managing the list at microbio-owner@net.bio.net When replying, please edit your Subject line so it is more specific than "Re: Contents of Microbio digest..." Today's Topics: 1. Re: Microbio Digest, Vol 52, Issue 2 (jorge1907@aol.com) 2. Re: USP Microbial Limits Prep Test (Christina Benson) (Kate Hawley) 3. Can any body give some suggetion about growing biofilm on RO membrane (sajeesh k) 4. hai (subhash subhash) ---------------------------------------------------------------------- Message: 1 Date: Wed, 09 Sep 2009 17:24:35 -0400 From: jorge1907@aol.com Subject: [Microbiology] Re: Microbio Digest, Vol 52, Issue 2 To: microbio@oat.bio.indiana.edu Message-ID: <8CBFFAB1067752F-2DD8-3A49A@webmail-m069.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" What dio you mean "prep" test??? Neutralization is intended to allow detection of viable by not growing bugs. -----Original Message----- From: microbio-request@oat.bio.indiana.edu To: microbio@magpie.bio.indiana.edu Sent: Wed, Sep 9, 2009 1:05 pm Subject: Microbio Digest, Vol 52, Issue 2 Send Microbio mailing list submissions to microbio@net.bio.net To subscribe or unsubscribe via the World Wide Web, visit http://www.bio.net/biomail/listinfo/microbio or, via email, send a message with subject or body 'help' to microbio-request@net.bio.net You can reach the person managing the list at microbio-owner@net.bio.net When replying, please edit your Subject line so it is more specific than "Re: Contents of Microbio digest..." Today's Topics: 1. USP Microbial Limits Prep Test (Christina Benson) ---------------------------------------------------------------------- Message: 1 Date: Tue, 8 Sep 2009 16:52: 12 -0600 From: "Christina Benson" Subject: [Microbiology] USP Microbial Limits Prep Test To: Message-ID: <24031E4AE32244F98F946FA60DED23FC@NLNT> Content-Type: text/plain; charset="us-ascii" Hi there, I was wondering if you would be willing to share the SOP you wrote on how to perform the prep test? I am having a hard time figuring it out. I also think it is kind of an oxymoron to be testing antimicrobial stuff. Antimicrobial is good, right? Why ruin it by neutralizing? Thanks for your time, Christina Benson ------------------------------ _______________________________________________ Microbio mailing list Microbio@net.bio.net http://www.bio.net/biomail/listinfo/microbio End of Microbio Digest, Vol 52, Issue 2 *************************************** ------------------------------ Message: 2 Date: Wed, 9 Sep 2009 16:42:53 -0700 (PDT) From: Kate Hawley Subject: [Microbiology] Re: USP Microbial Limits Prep Test (Christina Benson) To: microbio@magpie.bio.indiana.edu Message-ID: <787873.67102.qm@web52907.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Christina:In testing antimicrobials, neutralization should always be carried out to define the testing period. ?For example, you are looking at bleach and wonder how many bacteria are killed in a 15 second contact period. ?You expose the bacteria to the bleach for 15 seconds, then recover them in neutralizer. ?This stops the action of the bleach, the test period is defined as 15 seconds. ?Then plate the solution and wait for the 24 to 48 hours to see how many organisms survived and calculated your reduction from the inoculum (or zero time) organism count. ?If you had recovered the organisms in phosphate buffer or water, the bleach would still be acting on the organisms over the period required to enumerate the bacteria and your test period now is undefined or at best the whole 24 to 48 hours it takes to look at the plates. ?Regardless of the antimicrobial, whether fast acting like bleach/disinfectants or slower like silver or triclosan, neutralization is required for understanding kill times. ?Common neutralizers include letheen broth, D/E broth, and SCDLP, but you need to determine the appropriate neutralizer for your antimicrobial. Katherine Harrell Hawley Message: 1 Date: Tue, 8 Sep 2009 16:52:12 -0600 From: "Christina Benson" Subject: [Microbiology] USP Microbial Limits Prep Test To: Message-ID: <24031E4AE32244F98F946FA60DED23FC@NLNT> Content-Type: text/plain;??? charset="us-ascii" Hi there, I was wondering if you would be willing to share the SOP you wrote on how to perform the prep test?? I am having a hard time figuring it out.? I also think it is kind of an oxymoron to be testing antimicrobial stuff. Antimicrobial is good, right?? Why ruin it by neutralizing? Thanks for your time, Christina Benson ------------------------------ Message: 3 Date: Thu, 10 Sep 2009 02:06:58 -0700 From: sajeesh k Subject: [Microbiology] Can any body give some suggetion about growing biofilm on RO membrane To: microbio@magpie.bio.indiana.edu Message-ID: <1e0abc6a0909100206o190e83feg839e8cf4e58d013@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Respected Reserchers, I am trying to set a chemostat experimental set up using CDC reacter for growing Aeromonas hydrophila biofilm on Reverse osmosis membrane. Can any body give suggetion about the strength of LB media sutable for the experimnet and the dilution facter.Also how long i shuld run the chemostat to get a robust biofilm on RO surface. Thaking you in advance, Sajeesh ------------------------------ Message: 4 Date: Wed, 9 Sep 2009 23:32:50 -0700 From: subhash subhash Subject: [Microbiology] hai To: Microbio@magpie.bio.indiana.edu Message-ID: <666de2570909092332w5b3f5392rd562894942c21257@mail.gmail.com> Content-Type: text/plain; charset=I SO-8859-1 sirs and madams here i have one problem with cy3 cy5 fluorescent images they show only light imaging cntrovercy during epifluorescent microscopic observation.i cont differentiate the cy5 images from cy3 images. cy5 images are not visualised under this epifluorescent microscope, so please help to me reduce such problems during visualisation. if possible explain with diagrams. with regards subhash.g.venkat ------------------------------ _______________________________________________ Microbio mailing list Microbio@net.bio.net http://www.bio.net/biomail/listinfo/microbio End of Microbio Digest, Vol 52, Issue 3 *************************************** From joseba.mesa from gmail.com Sat Sep 12 13:06:45 2009 From: joseba.mesa from gmail.com (Jose Antonio Mesa Gonzalez) Date: Sat Sep 12 18:47:13 2009 Subject: [Microbiology] Last Call for Abstract Submission - BioMicroWorld2009 Conference, Lisbon (Portugal), 2-4 December 2009 Message-ID: III International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2009), Lisbon (Portugal), 2-4 December 2009 http://www.formatex.org/biomicroworld2009 Extended Deadline for Abstract Submission: 29 September 2009 / Extended Deadline for Early Registration: 18 September 2009 Dear colleague, You are still in time to submit abstracts to the BioMicroWorld2009 Conference in order to share your research in the field of applied microbiology. EXTENDED DEADLINE FOR ABSTRACT SUBMISSION: 29 SEPTEMBER If you are interested in submitting an abstract: http://www.formatex.org/biomicroworld2009/abstractsubmission.php The Conference will be structured in 8 general sessions and 10 special sessions: General Sessions of BioMicroWorld2009 - Agriculture, Soil, Forest Microbiology - Analytical and Imaging Techniques. Microscopy - Environmental, Marine, Aquatic Microbiology. Geomicrobiology - Food Microbiology - Industrial Microbiology - Future Bioindustries - Medical & Pharmaceutical Microbiology - Methods - Quantitative Models and Bioinformatics in Microbiology - Microbial Physiology, Metabolism and Gene Expression Special Sessions of BioMicroWorld2009 - Antimicrobial Surfaces - Bacterial derived antimicrobial toxins - Biofilms - Bioremediation - Biotechnologically Relevant Enzymes and Proteins - Microarrays in Microbiology - Microbial Biosensors (transducers, recognition) - Microfactories - Microbial Production of Chemicals and Pharmaceuticals - Single-cell analysis of microbes - Synthesis of nanomaterials (nanoparticles, nanowires...) by microorganisms Key Dates - 18 September 2009: Extended deadline for early registration - 29 September 2009: Extended deadline for abstract submission - 28 October: Notification of definitive presentation mode (for participants whose first option is oral presentation mode) - 11 November 2009: Deadline for registration Accepted abstracts/proposals will be presented in the Conference and published in the Book of Abstracts. Besides, they will also be considered for long papers, which could be published either in the special issues of the journals with which the Conference will agree their publication, or in the Conference Proceedings Book that will be published in 2010 by World Scientific Publishing Group, which is one of the world=92s leading academic publishers that will ensure an international distribution of the BioMicroWorld2009 Proceedings. Plenary Lecturers Dr. Hermann Heipieper, from the Helmholtz Centre for Environmental Research =96 UFZ in Germany, will present the lecture titled =93Microbial Adaptation to Toxic Organic Solvents=96 Mechanisms and Biotechnological Applications=94 Prof. Dr. Stefan D=FCbel, from the Institute for Biochemistry and Biotechnology, Technical University of Braunschweig in Germany, will expound on "Antibodies from Bacteria" Virtual Participation In addition to the oral and posters presentations, a Virtual Participation mode has been established for those researchers unable to attend the Conference personally. These participants will be requested to send "virtual papers", which will be exhibited and discussed at the online platform for virtual participation. These papers can be prepared using Power Point, Flash or similar software, or video recordings. Please consult the conference website for details. Full details about the conference including registration, accommodation, venue, submission instructions and Lisbon useful information can be consulted on the conference website: http://www.formatex.org/biomicroworld2009 For any doubts or questions, please do not hesitate to contact us at biomicro2009@formatex.org BioMicroWorld2009 Secretariat Formatex Research Center e-mail: biomicro2009@formatex.org http://www.formatex.org/biomicroworld2009 Phone: +34 924 25 86 15 Fax: +34 924 26 30 53 From jbdundas from gmail.com Sun Sep 13 21:36:25 2009 From: jbdundas from gmail.com (jitesh dundas) Date: Mon Sep 14 10:25:30 2009 Subject: [Microbiology] Request For Help Message-ID: <326ea8620909131936t4415537el137f7df1c2c257a@mail.gmail.com> Dear Sir/Madam, I am studying the cell organelles in uni-cellular & multi-cellular organisms for their thinking capabilities.I have some tests to perform & I would appreciate if some expert in (cell-biology) could help me in providing experimental data for these tests. The tests are very basic & may have been already done. I open to some suggestions on any new experiments for my research.. Please let me know if you need anything else from my side. Regards, Jitesh Dundas http://jiteshbdundas.blogspot.com From limbic_lesion from hotmail.com Wed Sep 16 17:19:46 2009 From: limbic_lesion from hotmail.com (N10) Date: Thu Sep 17 09:58:15 2009 Subject: [Microbiology] Re: Request For Help References: Message-ID: <1fGdndOV7LIZ-SzXnZ2dnUVZ8g-dnZ2d@bt.com> Hi Jitesh I was going to dismiss your post and request as rubbish because on first read it seemed nonsensical. However I read your blog and you seem like a very bright enthusiastic guy to me and I really do wish luck and success in your career this statment : "I am studying the cell organelles in uni-cellular & multi-cellular organisms for their thinking capabilities" doesnt make sense what do you mean by "thinking". ? Regards Limbic "jitesh dundas" wrote in message news:mailman.952.1252941915.21502.microbio@net.bio.net... > Dear Sir/Madam, > > I am studying the cell organelles in uni-cellular & multi-cellular > organisms for their thinking capabilities.I have some tests to perform > & I would appreciate if some expert in (cell-biology) could help me in > providing experimental data for these tests. > > The tests are very basic & may have been already done. I open to some > suggestions on any new experiments for my research.. > > Please let me know if you need anything else from my side. > > Regards, > Jitesh Dundas > http://jiteshbdundas.blogspot.com > From finch.enteract from sbcglobal.net Tue Sep 22 08:53:53 2009 From: finch.enteract from sbcglobal.net (Deirdre Sholto Douglas) Date: Tue Sep 22 10:39:17 2009 Subject: [Microbiology] Re: Can any body give some suggetion about growing biofilm on RO membrane In-Reply-To: References: Message-ID: <7hs37mF2t6e9vU1@mid.individual.net> sajeesh k wrote: > Respected Reserchers, > I am trying to set a chemostat experimental set up using CDC reacter for > growing Aeromonas hydrophila biofilm on Reverse osmosis membrane. > > Can any body give suggetion about the strength of LB media sutable for the > experimnet and the dilution facter.Also how long i shuld run the chemostat > to get a robust biofilm on RO surface. Not to be nasty, but the entire purpose of experimentation is to try variations and find out what works on your own... if someone tells you what or how to do it, you're not de- signing an experiment, you're following a protocol. Deirdre From limbic_lesion from hotmail.com Wed Sep 30 18:26:08 2009 From: limbic_lesion from hotmail.com (N10) Date: Wed Sep 30 19:29:06 2009 Subject: [Microbiology] Re: Rising Plague References: Message-ID: You would do better to go get a Doctorate in Pharmacology or Microbiology and do something about this problem you percevive than actually prophiting from writing about it. N10 "sbm" wrote in message news:W9QH9LTC40076.9594328704@reece.net.au... > "Rising Plague: The Global Threat from Deadly Bacteria and > Our Dwindling Arsenal to Fight Them" by Brad Spellberg > (Amazon.com: http://xrl.us/RisingPlague ) > > (M&C) - Meant as a serious wakeup call to professionals and > laypersons alike, this is a well researched yet > approachable examination of a frightening future as the > adaptability of bacteria threatens to overtake humanity's > ability to create effective antibiotics. As drug companies > continue to focus on the more lucrative markets of erectile > dysfunction or overactive bladders, the number of new > antibiotics under development has dwindled to a pitiful > few. This, at a time when numerous strains of bacteria have > developed alarming resistance to every antibiotic currently > available and kill over 100,000 Americans annually.. > > Continued: http://xrl.us/RisingPlague2 > > >