[Microbiology] Sterilised LB media getting some medicine smell, while I was growing E.coli culture

[Hiranya Roychowdhury]

Medicine smell?  Not a very scientific description since olfaction is somewhat subjective!



Hiranya S. Roychowdhury, Ph.D.
Associate Professor
Health & Public Services
NMSU-Dona Ana Community  College
575 527 7725 (office)

________________________________________
From: microbio-bounces from oat.bio.indiana.edu [microbio-bounces from oat.bio.indiana.edu] on behalf of sudheer sangeetham [sudheer.pbm07 from gmail.com]
Sent: Thursday, September 22, 2011 10:14 AM
To: microbio from magpie.bio.indiana.edu
Subject: [Microbiology] Sterilised LB media getting some medicine smell, while I was growing E.coli culture

Hi Guys
I am curious to know one thing that, I sterilised the LB media (which was
little more diluted). I inoculated the cultures with suitable antibiotics
and while I was measuring the OD of bacteria I could smell some medicine was
there from the media, I really dont understand why it was like that, but not
like earlier. After the OD was 0.55, i induced with IPTG but unfortunately i
could nt able to express the protein.

The only mistake I did was LB media was little diluted, except this, I think
I have done everything right...

With curiosity, I would like to know what could be the problem? If any one
has any idea......

Thanks in advance



--
Sudheer Babu.S
Ph.D student
Institute of Biochemistry
Biological Research Center
Szeged,Hungary.
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