Distinguishing stringent from relaxed?
mbpln at s-crim1.dl.ac.uk
Wed Jan 25 13:50:39 EST 1995
I am in the process of transduing a Tn10 relA mutation from one strain of
Salmonella into another. Does anyone have a handy way of scoring the
relA-negative phenotype, so that I can check that I really do have a relA
mutation in the second strain? Cahel & Rudd in Neidhardt's E. coli & S.
typhimurium book mention SMG medium. Is this the best way of
distinguishing stringent from relaxed strains?
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