[Microbiology] Problem with cultivation on anaerobic solid media
(by limbic_lesion from hotmail.com)
Mon Mar 24 15:06:32 EST 2008
A silica gel envelope helps
"Mad Twins Dad" <sandro_photo from yahoo.com> wrote in message
news:mailman.1195.1206380447.2451.microbio from net.bio.net...
> Luzan Tatiana wrote:
>> In my lab we use hungate bottles only for the liquid anaerobic media and
>> layer solid media. We have to do inoculations on solid meida only using
>> Petri dishes in the anaerobic jar supplemented with gas-packs or
>> atmosphere anaerobic chamber. THere is a problem in the chamber: the cag
>> mixture we use is 10% Hydrogen, 15% CO2 and Nitrogen as the rest. While
>> incubating plates inside, the media dries up very quickly and an
>> condensation is forming! IT is almost a lake of water there.
>> IS there any product which would absorb an acess condensate?
>> Thanks a lot
>> Tanya Luzan
>> Research Assistant
>> Department of Biology
>> University of Louisiana at Lafayette
>> Phone (Office): 482-5056
>> Microbio mailing list
>> Microbio from net.bio.net
> Tanya, I would agree with using sterile selica,
> another option you have - just dry your plate for a while.
> I guess it will be rather enough to leave 1.5% agar plates overnight.
> PS I could not reach your email box, sorry.
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