From kdevik from gmail.com Mon Aug 3 13:38:15 2009 From: kdevik from gmail.com (Kanchanadevi k) Date: Mon Aug 3 14:28:47 2009 Subject: [Microbiology] restriction enzyme to digest 16srRNA pcr product Message-ID: <8b3d015d0908031138t1a687e9emf7bd78538dd47ce@mail.gmail.com> hi everyone i need to digest 16srRNA pcr product with restrication enzyme can any one tell me commonly used enzyme for 16srRNA pcr product. actually i have isolated around 50 strains of unknown bacterial sample and i did 16srRNA amplication with universal primer 1492R, 27F now i just wanted to see whether these bacterial strain are same or different for further studies for i wanted to digest them with restriction enzyme so can any one tell me which enzyme i can use. thank u so much -- K.Kanchana Devi, Research Scholar, From blackhole from abuse.plus.com Tue Aug 4 10:44:46 2009 From: blackhole from abuse.plus.com (Duncan Clark) Date: Tue Aug 4 11:44:03 2009 Subject: [Microbiology] Re: restriction enzyme to digest 16srRNA pcr product References: Message-ID: Historians believe that in newspost on Tue, 4 Aug 2009, Kanchanadevi k penned the following literary masterpiece: >i need to digest 16srRNA pcr product with restrication enzyme >can any one tell me commonly used enzyme for 16srRNA pcr product. > >actually i have isolated around 50 strains of unknown bacterial sample >and i did 16srRNA amplication with universal primer 1492R, 27F >now i just wanted to see whether these bacterial strain are same or >different for further studies >for i wanted to digest them with restriction enzyme >so can any one tell me which enzyme i can use. You will have to identify an enzyme that gives you enough fragments to see if the isolates are similar or not. One cannot suggest a particular enzyme as it will depend upon the sequence of your 16S. All I can suggest is that you try a few 4bp recognition enzymes i.e. Hae III, Hpa II, Sau3AI, Hinf I, Taq I, BstU I, Hha I etc. Pick the one that works best for you. Duncan -- I love deadlines. I especially like the whooshing noise they make as they go flying by. Duncan Clark GeneSys Ltd. From micromurugan74 from gmail.com Fri Aug 7 01:44:03 2009 From: micromurugan74 from gmail.com (murugan) Date: Fri Aug 7 08:44:23 2009 Subject: [Microbiology] NTG mutagenesis protocols Message-ID: <000501ca172a$79967810$6cc36830$@com> Pl send copy of protocol From Heather.Vincent from manchester.ac.uk Thu Aug 13 13:41:59 2009 From: Heather.Vincent from manchester.ac.uk (Heather Vincent) Date: Thu Aug 13 17:04:08 2009 Subject: [Microbiology] Introduction to Bioinformatics, October 2009 Message-ID: <4A845E77.7020006@manchester.ac.uk> Bioinformatics meets microbiology in many areas, including medicine, public health, agriculture and biodiversity. This introductory online course will provide valuable professional development for microbiologists working in a wide range of different areas. Our distance courses are delivered in a Virtual Learning Environment, which allows us to extend the classroom into the web. We use a range of tools to support our online teaching activities. Each course, which is paced to suit those in full-time employment, runs over 16 teaching weeks. Teaching and learning are focussed around tutor-supported exercises. In this course, participants work together on the interpretation of their results, before receiving feedback from the course tutor. Participants who wish to be assessed for credits at Masters level should complete additional independent research for assessment Week 1 Introduction to distance learning Bioinformatics as a knowledge-based discipline Weeks 2 and 3 Introduction to the sequence databases Quality, redundancy and annotation Sequence retrieval exercise and group discussion Weeks 4 and 5 Nucleic acid sequence analysis Weeks 6 and 7 Independent research and assessment Weeks 8 and 9 Sequence alignment and database search 1. use of BLAST and FASTA, choosing suitable options; 2. interpretation of the results. Weeks 10 and 11 Protein function prediction. Weeks 12 and 13 Protein structure prediction Practical exercise and group discussion Weeks 14 to 16 Independent research and work on the second assessment You will find information on all our courses, including fees and a link to the online application form, here : http://octette.cs.man.ac.uk/bioinformatics/index.html If you have any questions, or need advice on the module options, please contact Heather.Vincent from manchester.ac.uk