From ktseden from gwdg.de Fri Jul 3 06:09:20 2009 From: ktseden from gwdg.de (ktseden@gwdg.de) Date: Fri Jul 3 11:34:09 2009 Subject: [Microbiology] PBS2 pahge Message-ID: <32470.134.76.70.252.1246619360.squirrel@mailbox.gwdg.de> Dear all, does someone have an idea where to find Bacillus subtilis phage PBS2 (the clear-plaque mutant of PBS1 phage). BGSC does not have it. Thanks Haliunaa From jeyankarunanithi from gmail.com Sat Jul 4 08:39:52 2009 From: jeyankarunanithi from gmail.com (jeyannathann) Date: Sat Jul 4 13:18:53 2009 Subject: [Microbiology] Quorum Sensing Message-ID: Hi people, I am a 3rd year student of B.Tech Industrial Biotechnology.Am deeply interested in Quorum Sensing.I want to know more developments in your institutions and looking for chances of collaboration.Can you please alert me with current developments and innovations.Am open to all thoughts.You can contact me through jeyankarunanithi@gmail.com Thanks, Jeyan. From jeyankarunanithi from gmail.com Sat Jul 4 09:02:54 2009 From: jeyankarunanithi from gmail.com (jeyannathann) Date: Sat Jul 4 13:18:57 2009 Subject: [Microbiology] Re: Bacterium candidodestruens References: Message-ID: <6f05ddce-e1d2-4668-a3b3-5d493fdc34f9@y28g2000prd.googlegroups.com> On May 27, 12:15?pm, "Bumble B. Boy" wrote: > Hi all, > I have run across a rather obscure reference to a bacterium isolated in > 1956 that attacks Candida albicans, and I wish to obtain it. > Akiba, T and Iwata, K. On the destructive invasion of a new species, > "Bacterium candidodestruens" into Candida cells. 1954. Japanese Journal of > Experimental Medicine. 24(3) pp.159-166 > I have sent a letter to the University of Tokyo, Dept. of > Bacteriology(sic), but I suspect the authors may be retired or deceased. I > have checked ATCC but it was never deposited there. Does anyone have this > bacterium (pigmented gram negative ?rods) or know how I can go about > locating it? Please e-mail replies since I do not regularly read this > newsgroup. Thanks! > > Allen > > ### > > Dear Allen, > > Did you ever get an answer? I saw the reference also in > F.C. Odd's book "Candida and Candidosis: A Review a Bibliography" > 2nd Edition. Let me know what you came up with. > > He thought perhaps from their description that it was > probably a Serratia species. > > Later, > > Clarke Dear clarke, Did you came across Candida destructing bacteria anywhere else.I am also in search for it.Can you please reply about this.Expecting your reply.my mail id jeyankarunanithi@gmail.com Thanks Jeyannathann Karunanithi From vasu.nanobiotech91 from gmail.com Sun Jul 19 13:06:06 2009 From: vasu.nanobiotech91 from gmail.com (shrinivas Dengeti) Date: Sun Jul 19 13:40:02 2009 Subject: [Microbiology] Suggestion for primer designing for expression Message-ID: Hi friends.. I am working with pET vector system. for expression of the enzme in E.coli BL21. I have to design primer with Nco1 site in forward primer. so can anybody will suggest me about the placing of ATG site of ORF...whether it should be in the primer's R.E site or else again i have to add in the primer aftre the R.E site. waiting for suggetion from scientific community From akiu0325 from hotmail.com Wed Jul 22 22:35:03 2009 From: akiu0325 from hotmail.com (Akihiro Ueda) Date: Thu Jul 23 01:35:15 2009 Subject: [Microbiology] Suggestion for primer designing for expression Message-ID: Restriction site for NcoI has ATG=2C=20 so you can use this ATG in frame to start codon of your gene. Make sure your gene does not have NcoI site. -QQA _________________________________________________________________ Windows Live=99 Hotmail=AE: Celebrate the moment with your favorite sports = pics. Check it out. http://www.windowslive.com/Online/Hotmail/Campaign/QuickAdd?ocid=3DTXT_TAGL= M_WL_QA_HM_sports_photos_072009&cat=3Dsports= From AHemberger from paddocklabs.com Thu Jul 23 08:22:36 2009 From: AHemberger from paddocklabs.com (Amber Hemberger) Date: Thu Jul 23 12:27:03 2009 Subject: [Microbiology] Microbiological Update Message-ID: <69FBF0D4C10B264A9093C57FA65707C12EA987320A@usnhmms1001.paddocklabs.com> I'm interested in the Microbiological Update. Where can I get more information? Thank you, Amber Hemberger Microbiology Supervisor Paddock Laboratories, Inc ahemberger@paddocklabs.com ________________________________ This e-mail message is intended for use only by the named addressee(s) and may contain information that is confidential and/or privileged material. If you are not the intended recipient please notify the sender by reply e-mail immediately and permanently delete the original and any copies of this email and any attachments without printing, reading copying or forwarding. Use, disclosure or reproduction of any information in this e-mail by anyone other than the intended recipient(s) is strictly prohibited. Paddock Laboratories, Inc. is not liable for any loss or damage arising in any way from transmission of the message or use of its attachments. This email may contain links to third party web sites or information. Paddock is not responsible for the content or privacy policies of such other sites and the existence of a link in this email does not imply an endorsement of the linked web site or the goods or services offered therein. From egillock from fhsu.edu Thu Jul 23 13:06:58 2009 From: egillock from fhsu.edu (egillock@fhsu.edu) Date: Thu Jul 23 14:10:11 2009 Subject: [Microbiology] Re: Microbio Digest, Vol 50, Issue 5 In-Reply-To: <200907231704.n6NH4jp21379@net.bio.net> References: <200907231704.n6NH4jp21379@net.bio.net> Message-ID: Hello, Does anyone know of a method to quickly distinguish Bacteria from Archaea. Differential media, PCR, or other biochemical methods would be very helpful. Thanks, Eric egillock@fhsu.edu ----------------------------------------------- Eric T. Gillock, Ph.D. Associate Professor Department of Biological Sciences Fort Hays State University Hays, KS 67601 ----------------------------------------------- From kdevik from gmail.com Fri Jul 24 12:23:45 2009 From: kdevik from gmail.com (Kanchanadevi k) Date: Fri Jul 24 14:22:10 2009 Subject: [Microbiology] deletin mutation and phenotype expression Message-ID: <8b3d015d0907241023k3ed9cc1bydf8d1c4bc49e6de7@mail.gmail.com> hi everyone There is one mutant bacterial strain which is mutated by deletion mutation of particular gene even after deletion mutation it's reported that this mutant strain can express that phenotype in minimal amount. so can any one tell me how is this possible the gene responsible for particular metabolite is deleted but still it's produces minimal amount of that metabolite. kanchan From mibrahim from minapharm.com Thu Jul 30 02:23:03 2009 From: mibrahim from minapharm.com (Mahmoud Ibrahim) Date: Thu Jul 30 11:09:06 2009 Subject: [Microbiology] Transformannt Yeast Cell Bank Message-ID: <9DEE5B292A34FE4C86D315BFF28EB80F2854506353@minapharm-mail.minapharm.net> Hi, I have a recombinant yeast strain on a petri dish that I wish to preserve. Does anyone have a protocol for performing a master cell bank for transformant yeast so that the viability of the cells and the stability of thhe transformation is maintained for a long time? I also have another question: if such a strain was kept on an agar plate for more than three months, will it loose its viability and will the transformation become unstable? Thanks a lot for your help in advance! Best Regards, Mahmoud Ibrahim From limbic_lesion from hotmail.com Thu Jul 30 17:11:37 2009 From: limbic_lesion from hotmail.com (N10) Date: Thu Jul 30 17:55:00 2009 Subject: [Microbiology] Re: deletin mutation and phenotype expression References: Message-ID: "Bob" wrote in message news:poak65dmp4c8d4ahj406nufpfqe28ghtqk@4ax.com... > On Fri, 24 Jul 2009 22:53:45 +0530, Kanchanadevi k > wrote: > >> hi everyone >> >> >>There is one mutant bacterial strain which is mutated by deletion mutation >>of particular gene >>even after deletion mutation it's reported that this mutant strain can >>express that phenotype in minimal amount. >> >>so can any one tell me >>how is this possible the gene responsible for particular metabolite is >>deleted but still it's produces minimal amount of that metabolite. > > > It would be best to consider the specific case. Can you give a > reference for this. > > But briefly in general... > > Is it really true? Are the analyses correct? Not always easy. > > If really true, then there may be a 2nd gene that can compensate. This > is the case for Lac+ strains of E coli derived after total deletion of > the original lactase. > > bob > An interesting question and a good reply by Bob but sadly no response from the originator...now I cant sleep ! N10 From arin280 from gmail.com Fri Jul 31 17:05:52 2009 From: arin280 from gmail.com (Arin280) Date: Mon Aug 3 12:44:16 2009 Subject: [Microbiology] Introducing the Global Directory of Doctoral Dissertations Message-ID: Dear Microbiology Researcher Doctoral dissertations help to advance knowledge and innovation. However, we have lacked one central location where the global research community can access these dissertations and some of the groundbreaking research they contain. With this in mind, MyNetResearch has created the Global Directory of Doctoral Dissertations containing details of thousands of dissertations. You can access them at: http://www.mynetresearch.com/Wiki/ Past and present doctoral students can also submit and update their dissertation details. The benefits include; (a) having your work cited, (b) having other researchers build upon your work, and (c) general recognition of your work. If you have completed or will soon complete your doctoral dissertation, please email us at admin@mynetresearch.com, with your: * Name * Doctoral Dissertation Title, * University, * Country, and * Year We will include your dissertation in the MyNetResearch Global Directory of Doctoral Dissertations. Currently, over 8,550 researchers from 95 countries and multiple research specialties use MyNetResearch for their research activities. Visit the #1 rated Global Research Management and Collaboration Portal=97 MyNetResearch today, at: https://www.mynetresearch.com/Signup/SignUp.aspx Sincerely, Bay Arinze, Ph.D., Founding Editor MyNetResearch, Empowering Collaboration=99 From bks from panix.com Fri Jul 31 18:12:34 2009 From: bks from panix.com (Bradley K. Sherman) Date: Mon Aug 3 12:44:23 2009 Subject: [Microbiology] Re: Introducing the Global Directory of Doctoral Dissertations References: Message-ID: In article , Arin280 wrote: > >http://www.mynetresearch.com/Wiki/ > All of the links on the left side are broken. --bks