[Microbiology] Re: can glucose and KCN be autoclaved together

John via microbio%40net.bio.net (by yjgent from verizon.net)
Thu Jul 21 19:44:03 EST 2011


On Jul 20, 12:20 am, Kanchanadevi k <kde... from gmail.com> wrote:
> Hi every one
> Is it possible to autoclave KCN with glucose containing minimal medium
>
> I read glucose carmelizes while autoclaving by reacting with amino acids.
> So, glucose is always autoclaved separately and added to the required medium
> after autoclaving.
>
> Therefore I have a doubt wheather glucose will have any reaction with KCN If
> I autoclave it together with the minimal medium?
>
> can u please tell if u have any experience regarding this.
>
> thank you so much in advance
>
> have great days
>
> --

I used to make small batches of the base media and autoclave it and
then keep them in the refrigerator until needed. I also had filter
sterilized KCN stored and would add the KCN to the base only when
needed. And yes it was back in the days when we mouth pipetted
everything! I actually felt safer doing it by mouth than trying to
manipulate some of the early pipette helpers!


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