rrumpf at magnus.acs.ohio-state.edu (Robert Rumpf) writes...
>>I have a question, more of a survey, really, for anyone doing phylogeny
>>studies. Are you sequencing both strands of whatever gene you are sequencing,
>>or just one and assume that's good enough?
>broe at aardvark.ucs.uoknor.edu (Bruce Roe) writes:
>To insure accuracy of each base, the "rule of three" should be followed.
>Sequence each base twice in one orientation and once in the other, as a
>minimum. I can think of no instance, except for sloppy science, that this
>rule should not be followed.
Is that what they do with the human cDNAs ... ??? :-j
| B.F. Francis Ouellette
| manager, yeast chromosome I project
| dept of biology, McGill university, Montreal, Qc, Canada
|francis at monod.biol.mcgill.ca