In article <1992Jun16.132204.21060 at sifon.cc.mcgill.ca>, francis at monod.biol.mcgill.ca writes...
>rrumpf at magnus.acs.ohio-state.edu (Robert Rumpf) writes...
>>>>I have a question, more of a survey, really, for anyone doing phylogeny
>>>studies. Are you sequencing both strands of whatever gene you are sequencing,
>>>or just one and assume that's good enough?
>>>>>>Bob
>>>>broe at aardvark.ucs.uoknor.edu (Bruce Roe) writes:
>>>To insure accuracy of each base, the "rule of three" should be followed.
>>Sequence each base twice in one orientation and once in the other, as a
>>minimum. I can think of no instance, except for sloppy science, that this
>>rule should not be followed.
>>>>>Bruce,
>>Is that what they do with the human cDNAs ... ??? :-j
>>>francis
I can only speak for what we are doing in my lab.
-bruce
broe at aardvark.ucs.uoknor.edu
