IUBio

Sequencing: Both strands or one?

Bruce Roe broe at aardvark.ucs.uoknor.edu
Tue Jun 16 06:32:00 EST 1992


In article <1992Jun15.185115.19849 at magnus.acs.ohio-state.edu>, rrumpf at magnus.acs.ohio-state.edu (Robert Rumpf) writes...
>I have a question, more of a survey, really, for anyone doing phylogeny 
>studies.  Are you sequencing both strands of whatever gene you are sequencing, 
>or just one and assume that's good enough?
> 
>Bob

To insure accuracy of each base, the "rule of three" should be followed.
Sequence each base twice in one orientation and once in the other, as a
minimum.  I can think of no instance, except for sloppy science, that this
rule should not be followed.

  - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
 \  Bruce A. Roe               Professor of Chemistry and Biochemistry /
 /  University of Oklahoma     INTERNET: BROE at aardvark.ucs.uoknor.edu  \
  - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  - - -



More information about the Mol-evol mailing list

Send comments to us at biosci-help [At] net.bio.net