In article <weixCKMEto.68v at netcom.com> weix at netcom.com (Patrick Weix) writes:
>This question and answer should probably be in Methods&Reagents but
>here goes:
> I had the same difficulty with resolving allelotypes of simple
>sequence length polymorphisms (SSLPs), some of which differ by just a
>few bases. The solution that was suggested to me was to run the
>samples on a Formamide/Urea Sequencing gel.
> For a 100ml tot vol use:
> 42g Urea
> 10ml 38%/2% Acyl/Bis
> 10ml 10xTBE
> 40ml deionized formamide
> H2O to 100mls.
>>Don't bother to prerun. Use 500microL of 10% APS and 75 microL of
>TEMED.
Just wanted to state my support for Formamide Urea gels as well.
Pete Rogan
Div. Genetics
Dept Pediatrics
Penn State Col of Med
Hershey PA
