Would someone be so kind as to disclose the current thinking about
calculating basal mutation rates for organisms. Specifically, I am
wondering about arguments against (or in support of) Drake's (1991)
estimate of spontaneous mutations with respect to the genome as
opposed to base pairs. Extremely specifically, I'm attempting to
nail down a good estimate of basal mutation rate in E. coli. So, do
I use a probabilistic method to estimate the basal rate of a gene or
region that lacks sequence data or is there an algorithm in existence
that accountts for A+T and G+C content, data from other regions, ect.
to provide an estimate? Thanks in advance for any help!
Drake, J. W. 1991. A constant rate of spontaneous mutation in DNA-
based microbes. PNAS. 88:7160-64.
higginss at vax.cs.hscsyr.edu