Greetings,
I'm thinking of devoting some valuable time to using RAPD techniques to
find molecular markers for a chromosome. I would greaty appreciate any
imput from people with experience.
Specifically:
1) What parameters (in both preparation of my specimen and
amplification) are most important to obtaining good and consistant results?
2) How many bands can I expect from a single decamer primer?
3) What about combining primers to expedite my search for a
useful marker ? What is the upper limit of number of primers to begin
with?
4) Will I have problems convincing people that my RAPD "marker"
isn't really an artifact ? What level of consistancy can be reasonably
expected?
Any imput will be greatly appreciated. Please reply via e-mail to:
Steve Dobson
dobson at mendel.berkeley.edu
Thank you very much,
Steve
