Katja Fechteler (kfecht at scan) wrote:
: Hi netters, my question is , which species is more related to
: hamsters: mice, rats or humans. I have to use an antibody against the
: protein PDI (protein disulphid isomerase). It is not cloned from
: hamsters, and antibodies are available only from mice, rats or human.
: Please send replies to my personal email
: adress:kfecht at scan.genetik.uni-koeln.de.: Thanks a lot for your help. Katja
A quick BLAST search with the mouse PDI (SwissProt:PDI_MOUSE)
suggests that all mammalian PDI are at least 91% identical (or so.
Hence, any polyclonal serum against one mammalian PDI is likely to
recognize the rest.
Hamsters are rodents, but I don't know offhand the resolution of
the rat-mouse-hamster trichotomy.
Good luck!
Keith Robison
Harvard University
Department of Cellular and Developmental Biology
Department of Genetics / HHMI
robison at mito.harvard.edu
