Katja Fechteler (kfecht at scan) wrote:
: Hi netters, my question is , which species is more related to
: hamsters: mice, rats or humans. I have to use an antibody against the
: protein PDI (protein disulphid isomerase). It is not cloned from
: hamsters, and antibodies are available only from mice, rats or human.
: Please send replies to my personal email
: adress:kfecht at scan.genetik.uni-koeln.de.: Thanks a lot for your help. Katja
Rat/mouse ca. 10-20 Myr
Mouse or rat/hamster ca. 30-60 Myr
hamster,mouse,rat/human ca. 80-120 Myr
In molecular terms, rat may be a little closer than mouse is to
hamster because mouse is suspected of having developed an increased
divergence rate. I doubt if the difference would be noticable with
Ab binding.
A polyclonal serum has a very good chance of working from either mouse
or rat, and a reasonable chance of working from human. Monoclonals
would be a crap-shoot.
Steve Hardies, Dept. of Biochemistry, Univ. of Texas HSC at San Antonio
Hardies at thorin.uthscsa.edu
