Hello,
We are currently in the process of sequencing a 1450 bp piece of the 16S
gene we have amplified from several microbes. We can get 500 to
600 bases of good sequence from either end, but the goal is to sequence
the whole fragment. We would like to avoid subcloning and use
internal primers to sequence the rest and were hoping for suggestions or
references.
Thanks in advance,
Tim Steppe
UNC-CH
tfs6030 at isis.unc.edu
