In article <3paf8k$cvf at due.unit.no>, Jan Sunde <jansunde at stud.unit.no> says:
>>Could anyone tell me anything at all about isolating DNA from feathers ?
>Methods, practical problems, etc ?
>Have you seen this article
TI- DNA EXTRACTION, AMPLIFICATION, AND DIRECT SEQUENCING
FROM HORNBILL
FEATHERS
AU- MORIN, PA;MESSIER, J;WOODRUFF, DS
NA- UNIV CALIF DAVIS,DEPT ANTHROPOL,DAVIS,CA,95616
UNIV CALIF SAN DIEGO,DEPT BIOL,LA JOLLA,CA,92093
UNIV CALIF SAN DIEGO,CTR MOLEC GENET,LA JOLLA,CA,92093
JN- JOURNAL OF THE SCIENCE SOCIETY OF THAILAND
PY- 1994
VO- 20
NO- 1
PG- 31-41
AB- Two methods for extracting DNA from feathers for non-invasive
multilocus genotyping of birds are described DNA was amplified by the
polymerase chain reaction and the double-stranded products were
sequenced directly without the need for cloning. One method uses a
single extraction step with Chelex as the extraction medium thus
reducing opportunities for contamination, and eliminating the need to
use organic solvents and proteinases. It constitutes a simple, fast and
relatively inexpensive method of DNA-level genotyping. Partial
mitochondrial cytochrome b sequences of 7 species of hornbill (Aves:
Bucerotidae) and a warbler are reported to demonstrate the method's
potential for phylogenetic and genealogical analyses involving
free-ranging birds and museum specimens.
