I believe you can use the probability equation from Sambrook:
N = ln(1-P)/ln(1-f), where P is the desired probability, f is the fractional
proportion of the genome in a single recombinant, and N is the
necessary number of recombinants (Clarke and Carbon, 1976).
eg. for a 99% probability of a 17 kb fragment in a mammalian genome of
3 X 10 E09 bp:
N= ln(1- 0.99) = 8.1 X 10 E05
ln( 1- [1.7 X 10 E04/ 3 X10 E09])
>>> BURMAN ADLAI J <fsajb4 at aurora.alaska.edu> 04/28/96 06:54pm >>>
I've been trying to figure out something that should be simple but I seem to be stuck.
Perhaps someone can help. I need to figure out what the probability of NOT finding a
fragment which is r bp long in a sequence which is n bp long. I can do this for
individual cases but I need a purely symbolic solution which would cover all cases.
This is all assuming a completely random sequence. If anyone happens to know off
the top of their heads what the solution is it would be grotesquely appreciated.